葡萄柚品种哈路比叶片离体再生体系的优化

摘要

为给葡萄柚的大规模育苗提供方法,以葡萄柚品种哈路比的叶片为外植体,在MS培养基中添加不同浓度配比的激素,探索其离体再生的优化体系.试验结果如下:培养基为MS+0.6 mg/L 6-BA+1.0 mg/L 2,4-D愈伤组织诱导率最高为78.57％,愈伤组织呈黄绿色,结构疏松,长势良好;MS+3.0 mg/L 6-BA+1.5 mg/L NAA是诱导愈伤组织分化不定芽的最佳培养基激素浓度配比,分化率最高为48％,不定芽分化快,长势良好;培养基MS+2.0 mg/L IBA有利于诱导生根,生根率达60.74％.初步建立了葡萄柚哈路比的离体再生体系.%In order to provide some methods for large-scale seedling in Citrus paradisi,using leaves of Hudson Foster as explants,regeneration system in vitro was explored in MS medium with different concentrations and proportion of hormones.The results were as follows:the medium of MS+0.6 mg/L 6-BA+1.0 mg/L 2,4-D was conducive to inducing callus,in which the highest induced rate was 78.57％,the callus was kelly and loose,and grew well;the medium of MS+3.0 mg/L 6-BA+1.5 mg/L NAA had the best hormone proportion for inducing adventitious buds from callus,differentiation rate was up to 48％,and the adventitious shoots had fast differentiation speed and grew well;the medium of MS+2.0 mg/L IBA was conducive to rooting,and rooting rate reached 60.74％.A regeneration system in vitro of Hudson Foster was established.