两益汤对哮喘豚鼠引喘潜伏期及TNF-α、IL-4水平的影响

摘要

Objective: To observe the effects of LiangYiTang on the latency period of asthm a and the levels of TNF-α and interleukine-4 (IL-4) in guinea pig, and explore the mechanism of preventing asthma. Method: A cute asthm atic models were established with them ixture of 2% acetylcholine chloride and 0.1% histam inephosphate to observe the influence of physiological saline (Group A), LiangYiTang (G roup B) and am inophylline (Group C) on the incubation period of asthma; chronic asthm atic models were built with ovalbum in challenge method. Expressions of TNF-α and IL-4 in the groups of physio logical saline(D), LiangYiTang(E) and dexam ethasone(F) were detected with enzyme-linked immunosorbent assay (ELISA). Result: Compared with the group of physiological saline, LiangYiTang could prolong the latency period of asthm a in guinea pig significantly (P<0.01), com parisons betw een LiangYiTang group and am inophylline group had no significantdifference(P>0.05);LtangYiTang could low erserum TNF-α of asthm atic guinea pig significantly (P<0.05) and level of IL-4 (P<0.01). Conclusion: LiangYiTang could delay the latency period of asthm a in asthm atic guinea pig, the function of inhibition to the inflam mation of respiratory tractm ightbe related to dow n regulation the expression of TN F-α and IL-4.%目的:观察两益汤对哮喘豚鼠引喘潜伏期和血清TNF-α和IL-4水平的影响,探讨其防治哮喘的机理.方法:采用2%氯化乙酰胆碱和0.1%磷酸组胺等体积混合液制备急性豚鼠哮喘模型,观察生理盐水(A组)、两益汤(B组)和氨茶碱(C组)对哮喘潜伏期的影响;以卵蛋白致敏法制备慢性豚鼠哮喘模型,应用酶联免疫吸附试验法测定生理盐水组(D组)、两益汤组(E组)和地塞米松组(F组)豚鼠血清TNF-α和IL-4的表达水平.结果:与生理盐水组相比,两益汤组能明显延长豚鼠哮喘潜伏期(P＜0.01),两益汤组与氨茶碱组相比无明显差异(P＞0.05);两益汤组能显著降低哮喘状态下豚鼠血清TNF-α(P＜0.05),并能显著降低哮喘状态下豚鼠血清IL-4的水平(P＜0.01).结论:两益汤有延长哮喘豚鼠引喘潜伏期的作用,其对哮喘呼吸道炎症的抑制可能与下调哮喘豚鼠TNF-α和IL-4的表达有关.