茴香枳术汤对粘连性肠梗阻模型大鼠血浆NO、DAO 的影响

摘要

Objective: To provide experimental evidence for the mechanism of HuiXiang ZhiZhuTang in treating adhesive intestinal obstruction through observing the effects of the decoction on nitric oxide (NO) contents and diamine oxidase (DAO) in rats with adhesive intestinal obstruction. Method: All 108 rats were randomly divided into normal group, model group, DaChengQiTang group, low, moderate and high dose groups of HuiXiang ZhiZhuTang. Except normal group, the models were established and given with corresponding medicine at the 8th day. Six rats were drawn blood from femoral each time at the third, fifth and seventh day after treating. NO content and DAO activity in the serum were detected with radioimmunoassay. Result: Compared with normal group, No contents and DAO activity in the model group were increased significantly (P<0.05); compared with the model group, NO content and DAO activity in DaChengQiTang group, low, moderate and high dose groups of HuiXiang ZhiZhuTang were decreased remarkably(P<0.05). Conclusion: HuiXiang ZhiZhuTang shows better effects in treating adhesive intestinal obstruction and the mechanism might be related to the protection of intestinal barrier.%目的:观察茴香枳术汤对粘连性肠梗阻大鼠血浆一氧化氮(NO)含量、二胺氧化酶(DAO)活性的影响,为茴香枳术汤对粘连性肠梗阻的作用机制提供实验依据.方法:将108 只SPF 级Wistar 大鼠随机分为正常组,模型组,大承气汤组,茴香枳术汤低、中、高剂量组.除正常组外,其余各组依法制备粘连性肠梗阻大鼠模型,并于造模后第8 天开始给予相应药物治疗,分别于治疗后第3、5、7 天,共3 个时间点,每时间点6 只大鼠股动脉取血,放免法测定血浆NO含量、DAO 活性.结果:与正常组比较,模型组血浆NO含量、DAO活性显著增加(P＜0.05);与模型组比较,大承气汤组,茴香枳术汤低、中、高剂量组血浆NO含量,DAO活性显著下降(P＜0.05).结论:茴香枳术汤具有良好的治疗粘连性肠梗阻的作用,其作用机制可能与保护肠屏障功能有关.