银柴胡提取物对兔脊髓慢性渐进性压迫后基质金属蛋白酶MMP-2、12的表达及神经功能的影响

摘要

Objective:To discuss the influence of YinChaiHu (Radix Stellariae) extracts on neurological func-tion, the expressions of matrix metalloproteinase-2, 12 (MMP-2, 12) of the rabbits suffering from chronic progressive oppressive spinal cord injury. Methods: The rabbits were randomized into the control group (group A) receiving physiological saline and the treatment group (group B) intervened by YinChaiHu extracts after spinal cord injury, the tissue was cut at different times, pathological changes of damaged spinal cord were observed by HE staining;the ex-pressions of MMP-12 by Real-Time PCR and MMP-2 by immuohistochemical staining; neurological function as-sessed by Tarlov scaling. Results:HE staining microscopy revealed that group B was lighter than group A obviously in histopathology of spinal cord. The expressions of MMP-2, 12 were found in both groups, group A was more than group B in the expressions of MMP-2, 12(P<0.05). Group B was superior to group A in the improvements of neuro-logical function. Conclusion:YinChaiHu extracts could effectively inhibit the expressions of MMP-2, 12, promote the recovery of neurological function of the rabbits suffering from chronic progressive oppressive spinal cord injury.%目的：探讨银柴胡提取物对兔脊髓慢性渐进性压迫后基质金属蛋白酶2、12（MMP-2、12）表达及神经功能的影响。方法：家兔随机分为脊髓损伤后应用生理盐水对照组(A组)和银柴胡提取物干预治疗组(B组)，损伤后不同时间取材,HE 染色观察损伤脊髓组织病理变化；免疫组化染色检测MMP-2及Real-Time PCR检测MMP-12的表达变化；采用Tarlov评分评价神经功能。结果：HE染色镜检发现脊髓组织病理学改变B组明显轻于A组。2组均发现MMP-2、12的表达，MMP-2、12表达A 组>B组(P＜0.05)。 B组神经功能改善优于A组。结论：银柴胡提取物可有效抑制家兔脊髓慢性渐进性压迫后MMP-2、12表达，促进神经功能恢复。