荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素-2和肿瘤坏死因子-α水平的变化

摘要

Objective To investigate the change of T lymphocyte subsets, interleukin (IL)-2 and tumor necrosis factor (TNF) -α in the tumor- bearing mice and patients with oral cancer receiving thermo-chemotherapy, and investigate the correlation among them. Methods After treatments, the expression of lymphocyte transformation index (LTI), IL-2 and TNF-a in the tumor-bearing mice were detected with methyl thiazolyl tetrazolium (MTT), the expression of IL-2 and TNF-a in the patients with oral cancer were detected with MTT, the expression of LTI, CD44 and CD8+ were detected with 3H-TdR incorporation. Results LTI, IL-2 and TNF-a of thermo-chemotherapy group (HP group) had no significant difference comparing with those of normal mice group (N group) CP>0.05), but which were significantly higher than those of chemotherapy group (P group) and no treatment group (NT group) (P<0.01). In clinical trials, the expression of LTI, CD4+, CD47CD8+, IL-2 and TNF-a on oral cancer patients after thermo-chemotherapy were significantly higher than those before thermo-chemotherapy CP<0.01). Conclusion After thermo-chemotherapy, the expression of LTI, IL-2 and TNF-a of tumor-bearing hosts are significantly improved, there is a significant correlation between IL-2 and T cell.%目的 探讨荷瘤鼠及口腔癌患者热化疗前后T淋巴细胞亚群、白细胞介素(IL) -2和肿瘤坏死因子(TNF)-α活性水平的变化,并初步探讨三者之间的相关性.方法 经热化疗处理后,采用甲基噻唑基四唑(MTT)法检测荷瘤鼠淋巴细胞转化指数(LTI)及IL-2、TNF-α水平,MTT法检测口腔癌患者IL-2、TNF-α水平,并采用3H-胸腺嘧啶核苷(3H-TdR)掺入法行淋巴细胞转化实验及CD4+、CD8+T细胞业群检测.结果 高温联合平阳霉素治疗组(HP组)荷瘤鼠LTI、IL-2和TNF-α活性与正常对照组(N组)荷瘤鼠差异无统计学意义(P＞0.05),而明显高于平阳霉素治疗组(P组)及不治疗组(NT组)(P＜0.01).临床实验中热化疗后口腔癌患者LTI、CD4+细胞数目及CD4+/CD8+比值明显提高,IL-2和TNF-α活性水平明显升高(P＜0.01).结论 荷瘤宿主的LTI及IL-2和TNF-α活性水平在热化疗后明显升高,IL-2和T细胞之间有明显的相关性.