目的：研究种植体周围炎条件下炎性微环境对颌骨成骨细胞生物学功能的影响。方法原代分离培养和纯化种植体周围炎来源和正常组织来源的人颌骨成骨细胞，取第3代细胞进行鉴定和检测，MTT法检测成骨细胞的增殖能力，实时定量聚合酶链反应法检测成骨细胞相关基因骨钙素（OCN）、Runx2、Ⅰ型胶原（ColⅠ）的表达， Westernblot法检测成骨细胞相关蛋白OCN的表达。结果从培养第4天起，种植体周围炎来源的成骨细胞的增殖活力明显低于正常组织来源的成骨细胞（P<0.05）。培养7d时，与正常组织来源的成骨细胞相比，种植体周围炎来源的成骨细胞相关基因OCN、Runx2、ColⅠ的表达均显著降低（P<0.05），相关蛋白OCN的表达也显著降低（P<0.05）。结论种植体周围炎的炎性微环境降低了颌骨成骨细胞的增殖及分化能力。%Objective To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts. Methods Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen Ⅰ (Col Ⅰ) mRNA levels were examined by real-time quantitative poly-merase chain reaction. OCN protein levels were determined by Western blot. Results After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue (P<0.05). After 7 d of culture, OCN, Runx2, and Col Ⅰ mRNA expression decreased (P<0.05). The OCN protein levels also decreased (P<0.05). Conclusion Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.
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