金属注射成型生物医用Ti-22Nb合金的表面形貌和细胞相容性

摘要

采用金属注射成型(MIM)工艺制备Ti-22Nb(质量分数,％)和商业纯钛(CP-Ti)参比样,并选取电弧熔炼后抛光的Ti-22Nb作为另一种参比样.采用激光共聚焦显微镜、扫描电子显微镜和酶联免疫检测仪检测每组样品的表面拓扑结构和细胞相容性.结果显示,MIM工艺可以得到微米级粗糙度,而且采用混合元素粉而不是单一钛粉作为原料可以获得更高的表面粗糙度和比表面积.所有测试样品都不存在细胞毒性.人原代细胞在MIM Ti-22Nb样品表面(特别是在闭孔附近)的吸附效果更好.%Metal injection molding (MIM) was applied to fabricating Ti-22Nb (mass fraction, ％) and commercially-pure Ti (CP-Ti, selected as reference) discs. As references, arc-melted and polished Ti-22Nb discs were employed. The surface topography and cytocompatibility were comparatively assessed on each configuration by microscopic analysis using confocal laser scanning microscopy and scanning electron microscopy and adhesion and viability tests. The results reveal that micron-scale roughness could be obtained via MIM process, and using blended Ti and Nb elemental powders instead of only Ti powder as raw materials leads to much higher surface roughness and surface area ratio. None of the three materials shows cytotoxicity, and the adhesion of human primary cells seems to be increased on the MIM Ti-22Nb specimens, especially around the closed-pores on the surface.