Tb/Mg掺杂对羟基磷灰石纳米颗粒基因载体理化特性的影响

摘要

Transfection efficiency of hydroxyapatite nanoparticles (HAnps) is relative to the particle size, morphology, surface charge, surface modifier and so on. This study prepared HAnps with doped Tb/Mg by hydrothermal synthesis method (HTSM) and investigated the effects of different Tb/Mg contents on the morphology, particle size, surface charge, composition and cellular endocytosis of HAnps. The results showed that Mg-HAnps possessed better dispersion ability than Tb-HAnps. With increasing doping content of Tb/Mg-HAnps, the granularity of Tb-HAnps increased, while that of Mg-HAnps declined. Both particle size and zeta potential of Mg-HAnps were lower than those of Tb-HAnps. 7.5% Mg-doping HAnps presented relatively uniform slender rod morphology with average size of 30 nm, while 10% Mg-doping HAnps were prone to agglomeration. Moreover, Mg-HAnps-GFP (green fluorescent protein) endocytosed by MG63 cells was dotted in the perinuclear region, while Tb-HAnps were more likely to aggregate. In conclusion, as gene vectors, Mg-HAnps showed enhanced properties compared to Tb-HAnps.%羟基磷灰石纳米颗粒(HAnps)基因载体的转染效率与粒径、形貌、表面电荷、表面改性等有关.本研究通过水热合成法制备掺杂Tb/Mg的HAnps,观察Tb/Mg掺杂量对HAnps形貌、粒径、表面电荷、成分和细胞胞吞作用的影响.结果表明,掺Mg组的分散性优于掺Tb组的分散性.增加掺杂量使Tb-HAnps的粒径增大,而Mg-HAnps的粒径减小.掺Mg组的粒径及zeta电位均低于掺Tb组的.掺Mg量为7.5%的HAnps平均粒径约30 nm,呈相对均匀的细长杆状,而掺Mg量为10%的HAnps易于团聚.被胞吞入MG63细胞的Mg-HAnps-GFP呈点状分布于核周区域并形成荧光圈,而Tb-HAnps易于团聚.因此,作为基因载体,Mg-HAnps明显优于Tb-HAnps.