不同消毒方法对TiO2涂层种植体抗菌性能的影响

摘要

目的：研究不同消毒方法对TiO2涂层种植体抗菌性能的影响。方法纯钛表面经0.5%质量分数的氢氟酸酸蚀后阳极氧化，制备TiO2涂层试样作为实验组，以未经处理的纯钛试样作为对照组。采用乙醇浸泡、高压蒸汽灭菌、紫外线照射3种消毒方法处理试样。观察试样表面形貌，测量试样表面粗糙度及消毒前后去离子水在试样表面接触角，评价试样的细菌黏附能力；采用薄膜密着法进行活菌计数，验证经3种消毒方法处理后，试样抑制金黄色葡萄球菌的功效；采用场发射扫描电镜观察3种消毒方法对细菌形态的影响。结果与对照组试样相比，实验组的表面粗糙度更大，对细菌黏附能力更强。经紫外线照射消毒后，试样表面接触角小于乙醇浸泡消毒和高压蒸汽灭菌。活菌计数和场发射扫描电镜观察显示，乙醇浸泡消毒后，试样表面活菌数最多，细菌形态较完整；高压蒸汽灭菌后，试样表面活菌数较少，细菌形态受到一定程度破坏；紫外线照射消毒后，试样表面活菌数最少，细菌形态破坏最完全。结论经紫外线照射消毒后，试样的抗菌性能最佳，高压蒸汽灭菌次之，乙醇浸泡消毒效果最差。%Objective To study the influence of different kinds of disinfection methods on antibacterial activity of TiO2 nanotubes. Methods TiO2 nanotubes were fabricated with 0.5%wt HF on pure titanium to acid-etching combined with an⁃ odization as experimental group. The pure Ti was control group. They were disinfected by three kinds of disinfection meth⁃ods, which were alcohol immersion, autoclaving and UV irradiation. The bacterial adhesion ability was evaluated by observ⁃ing the morphology of samples, measuring the roughness and contracting angle. The film contact method was used to evaluate the antibacterial activity of samples with Staphylococcus aureus (Sa) after different infection treatment. The bacterial morphol⁃ogy was observed by field emission scanning electron microscope (FE-SEM). Results The treatment group has higher sur⁃face roughness. The bacterial adhesion ability of experimental group was stronger compared with that of control group. The contracting angle of samples exposed to UV irradiation was smaller than that of alcohol immersion and autoclaving treatment group. The viable count and FE-SEM showed that there were more bacteria and more complete morphology on the sample surface after alcohol immersion. The viable count was less and bacterial morphology suffered some damage on the sample sur⁃face after high pressure steam sterilization. The least viable count and the most complete destruction of bacterial morphology were found after ultraviolet (UV) germicidal irradiation. Conclusion UV irradiation shows the best antibacterial activity, the second is autoclaving and the worst is alcohol immersion.