Objective To investigate intrapulmonary expression of ICAM - I mRNA and the relationship between the expression of ICAM - 1 and lung injury in ANP. Methods ANP was reproduced by retrograde injection of 3. 5 % sodium taurocholate. Animals were sacrified at 1,4,12 and 24 hours after induciton of ancreatitis. Intrapulmonary ICAM - I mRNA expression was assayed by reverse transcription - PCR. The following parameters were also measured:Serum amylase.histologic grading of lung injury and intrapulmonary myeloperoxidase (MPO). Results There was intrapulmonary overexpression of the ICAM -China Medical Abstracts (Surgery) 1 mRNA on the first hour and persisted up to 12 - 24 hours with the increase of serum amylase and intrapulmonary MPO and pancreatitis induction. Score of the lung injury correlated well with the expression of the ICAM- I mRNA and intrapulmonary MPO(r = 0.85,0. 85 and 0.96,all P0.05).Conclusion The expression of ICAM- I mRNA and pulmonary leucocyte infiltratiorK / was related with lung injury
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