Liposomes Containing Clodronate Attenuate Spleen Injury in the Rat Model with Severe Acute Pancreatitis

摘要

背景:Clodronate脂质体可选择性耗尽巨噬细胞，超顺磁性氧铁(SPIO)纳米颗粒可对巨噬细胞进行标记。在本研究中，探索clodronate-SPIO纳米颗粒-脂质体对重症急性胰腺炎(SAP)的保护作用。
　　方法:SPIO纳米颗粒采用热分解法合成。Clodronate-SPIO脂质体采用薄膜法制作。大鼠SAP的模型采用大胰腺被膜下的钠注射牛磺胆酸盐。大鼠慢慢注入SPIO-containing脂质体通过尾静脉（P组）。另外，大鼠T和C组注入clodronate-SPIO脂质体或生理盐水(2ml/kg)。血清淀粉酶(SAM)、白介素6(IL)和肿瘤坏死因子（TNF）-α采用ELISA分析。胰腺、脾脏病理改变HE染色并进行病理评分，TUNEL染色检测细胞凋亡。
　　结果:胰腺和脾脏的病理改变SAP模型大鼠注射clodronate-SPIO脂质体组比在SAP模型大鼠注射SPIO-containing脂质体明显减轻。血清淀粉酶水平、IL-6和TNF-α在SAP模型大鼠注射SPIO脂质体组高于C组，但在显著降低在clodronate-SPIO脂质体组(P＜0.01)。在脾脏凋亡率高clodronate-SPIO脂质体比SPIO脂质体在2和6h。
　　结论:Clodronate-containing脂质体可以把减轻SAP大鼠脾脏损伤、SPIO纳米颗粒可以作为示踪剂来检测在SAP大鼠脾脏损伤。

目录

摘要

ABSTRACT

CATALOGUE

Chapter 1

1．Introduction

2．Materials and Methods

2．1 Reagents

2．2 Preparation of SPIO／clodronate--SPIO nanoparticle--containing liposomes

2．3 Animal models and experimental grouping

2．4 Analysis of serum amylase，IL-6 and，TNF-α levels

2．5 Histopathological examinations of the pancreas and spleen pathological

2．6 Perl’S Prussian blue staining of spleen

2．7 TUNEL assays of spleen sections

2．8 Immunohistochemistry staining of spleen macrophages

2．9 Statistical analyses

3．Results

3．1 Characterization of SPIO and liposomes

3．2 Changes in the levels of serum amylase(SAM)，IL-6，and TNF-α

3．3 Gross changes，microscopic changes，and pathological scores of pancreases in all groups

3．4 Gross changes，microscopic changes，and pathological scores of spleens in all groups

3．5 Perl’S Prussian blue staining

3．6 Measurement of apoptosis by TUNEL assay

4．Discussion

5．References

Chapter 2 Advances in the relationship between macrophage and acute pancreatitis

Abstract

1．Introduction

2．Peritoneal maerophages with SAP

3 lung macrophages with SAP

4 liver macrophages with SAP

5 Renal macrophages with SAP

6 Spleen macrophage with sap

CONCLUSION

REFERENCE

ABBREVIATIONS

PUBLICATIoNS

ACKNoWLEDGMENT