痛血康胶囊治疗痛经的临床前药效学试验研究

摘要

OBJECTIVE To study the effect and mechanism of Tongxuekang Capsules on rats model of dysmen-orrhea.METHODS ①The dysmenorrhea model Rats were made by intraperitoneal injection of oxytocin.The effect of Tongxuekang capsules on the inhibiting writhing reaction in the rats caused by oxytocin 、the constriction of isolated uterine and Ovary coefficient in dysmenorrhea model rats and the effect on the content of β-EP in plasma、PGF2αin uterine contraction were studied.②The acute blood stasis model rats caused by epinephrine ice water bath ,th effect of Tongxuekang capsules on blood rheology were studies.③The analgesic effect of Tongxuekang capsules were stud-ied by writhing by acetic acid method and the anti-inflammatory action of Tongxuekang capsule were observed by the methods of mouse swelling ear caused by dimethylbenzene and cotton ball granuloma of rats.RESULTS Tongxuek-ang capsules could relieve the pain caused by oxytocin induced uterine intense contraction and inhibit the spontane -ously contractive activities of the smooth muscle in the rat uterine ( contraction amplitude and contractile frequency ) dramatically,could increase the content of β-EP in plasma,decrese the content of PGF2αin uterus tissue and the blood viscosity in blood stasis rats.Which also had anti-inflammatory effect.CONCLUSION Tongxuekang capsules has anti dysmenorrhea effect significantly.%目的：研究痛血康胶囊对痛经模型大鼠的疗效及作用机制。方法①大鼠腹腔注射催产素造成痛经模型，研究痛血康对催产素所致大鼠扭体反应的抑制作用、对痛经模型大鼠离体子宫收缩和子宫卵巢系数、血浆中β-EP、子宫匀浆中PGF2α含量的影响。②采用肾上腺素加冰水浴造成大鼠急性血瘀模型，研究痛血康对血液流变学的影响。③采用醋酸致痛扭体法研究痛血康的镇痛作用及采用二甲苯致小鼠耳廓肿胀及大鼠棉球肉芽肿法研究痛血康对炎症的抑制作用。结果痛血康对催产素所致大鼠子宫剧烈收缩引起的疼痛有明显缓解作用，对大鼠子宫平滑肌自发活动（收缩幅度与收缩频率）有明显的抑制作用，能够明显增加痛经模型大鼠血清β-EP含量，降低子宫组织中 PGF2α含量，降低血瘀大鼠血液粘度，还具有抑制炎症的作用。结论痛血康胶囊具有显著抗痛经作用。