HPLC法同时测定土荆芥中槲皮素、山奈素、异鼠李素的含量

摘要

OBJECTIVE To develop an HPLC method for determination of quercetin ,kaempferide and isorham-netin in Chenopodii ambrosioidis.METHODS The DIKMA Platisil ODS C18 (250mm ×4.6mm,5μm) column was used.The mobile phase was acetonitrile-0.3%phosphoric acid(38∶62) at a flow rate of 1.0mL・ min-1 and at 30℃of column temperature.The detection wavelength was 370nm.RESULTS The linear range for quercetin was 0.0559~3.3552μg,r=0.9999 (n=6).The average recovery was 104.82% and RSD was 1.83%.The linear range for kaempferide was 0.1112 ~6.6720μg, r =0.9999 ( n =6 ) .The average recovery was 100.82% and RSD was 2.43%.The linear range for isorhamnetin was 0.0128~0.7680μg,r=0.9999 (n=6).The average recovery was 99.49%and RSD was 2.55%.CONCLUSION This method is rapid and accurate ,which can be used to deter-mine the contents of quercetin ,kaempferide aod isorhamnetin in Chenopodii ambrosioidis .%目的：建立土荆芥中槲皮素、山奈素、异鼠李素含量的HPLC测定方法。方法采用 DIKMA Platisil ODS C18色谱柱（250mm ×4．6mm，5μm），流动相为乙腈－0．3％磷酸（38∶62），流速为1．0mL・ min －1，检测波长为370nm，柱温为30℃。结果槲皮素进样量在0．0559～3．3552μg与峰面积线性关系良好，r＝0．9999，平均回收率为104．82％，RSD为1．83％（n ＝6）；山奈素进样量在0．1112～6．6720μg与峰面积线性关系良好，r＝0．9999，平均回收率为100．82％，RSD为2．43％（n＝6）；异鼠李素进样量在0．0128～0．7680μg与峰面积线性关系良好，r＝0．9999，平均回收率为99．49％，RSD为2．55％（n＝6）。结论本法准确、重复性好，可用于同时测定土荆芥中槲皮素、山奈素、异鼠李素的含量。