HPLC法测定复合维生素B片维生素B1、维生素B2、烟酰胺的含量及含量均匀度

摘要

ABSTRACT:OBJECTIVE To establish HPLC determination of compound vitamin B,vitamin B1 ,vitamin B2 ,ni-acinamide content and content uniformity.METHODS The chromatographic column:Diamonsil C18 ( 250mm × 4.6mm,5μm),mobile phase:0.005mol· L-1heptane sodium sulfonate solution(including 0.5%glacial acetic acid and 0.5% triethylamine)-methanol (70∶30,V/V);Detection wavelength of 261nm,flow rate of 1.0mL· min -1 , sample quantity:20μL.RESULTS The concentration of vitamin B1 ,vitamin B2 ,and nicotinamide has a good linear relationship in the range of 2.65~66.2μg· mL-1 ,1.19~30.15μg· mL-1 ,7.83~196.2μg· mL-1 respectively, the average recovery were greater than 98%,RSD were less than 1.0%.CONCLUSION This method is simple, accurate,reliable and can be used as the quality control of vitamin B slice method.%目的：建立高效液相色谱法测定复合维生素B片维生素B1、维生素B2、烟酰胺的含量及含量均匀度。方法采用色谱柱：Diamonsil C18（250mm ×4.6mm，5μm），流动相：0.005mol· L －1庚烷磺酸钠溶液（含0.5％冰醋酸和0.05％三乙胺）-甲醇（70∶30，V／V）；检测波长为261nm，流速为1.0mL· min －1，进样量：20μL。结果维生素 B1、维生素 B2、烟酰胺浓度分别在2.65～66.2μg· mL －1、1.19～30.15μg· mL－1、7.83～196.2μg· mL －1范围内与峰面积线性关系良好，平均回收率均大于98％，RSD均小于1.0％。结论本方法简单、精确、可靠，可作为合维生素B片的质量控制方法。