牙龈卟啉单胞菌感染对人脐静脉内皮细胞表达IL-8影响的研究

摘要

目的 研究牙龈卟啉单胞菌(porphyrmonas gingivalis,P.g)感染时人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)表达趋化因子白介素-8(interleukin -8,IL -8)的变化,了解P.g对其趋化功能的影响.方法 建立P.g侵入HUVEC的体外模型,采用酶联免疫吸附法(ELISA)研究P.g381和P.g33277菌株感染HUVEC 6、24h后的培养上清液中IL-8的浓度.结果 ELISA结果显示当P.g381感染HUVEC6、24h和P.g33277感染HUVEC6、24h时,HUVEC分泌IL -8的水平明显升高(P＜0.01)；感染24h时P.g381促进HUVEC表达IL -8的水平高于P.g33277(P＜0.01).结论 P.g侵入HUVEC后可促进其表达IL -8,从而上调其趋化功能,可能与动脉粥样硬化(atherosclerosis,AS)的发生发展相关.%Objective To elucidate the ability of porphyrmonas gingivalis (P. g) to modulate the expression of chemotatic factor in-terleukin-8 (IL-8) and the chemotactic function in human umbilical vein endothelial cells ( HUVEC). Methods The model of infection of HUVEC by P. g was established in vitro. HUVEC were infected with either P. g381 or P. g-33277 ,then the expression of IL-8 in supernatant was tested by ELISA. Results The expression of IL-8 was signicantly upregualted in P. g381 -infected and P. g33277-infected HUVEC at 6 h and 24 h( P <0. 01). The upregualtion effect of P. g381 was significantly greater than P. g33277at 24 h( P < 0.01). Conclusions These data demonstrate that the infection of P. g can increase the expression of IL-8 and chemotactic function of HUVEC,which may be related to the development of AS.