孕期碘营养状态对子代大鼠成骨细胞的影响

摘要

目的：观察孕期碘营养状态的改变对大鼠仔鼠成骨细胞的的影响。方法雌性 Wistar 大鼠，交配妊娠后，取孕鼠20只按体质量随机分成对照Ⅰ组、对照Ⅱ组、碘缺乏组、碘过量组。自妊娠日起，对照Ⅰ组饲以普通饲料＋去离子水，对照Ⅱ组饲以缺碘饲料＋适碘去离子水，碘缺乏组饲以缺碘饲料＋去离子水，碘过量组饲以普通饲料＋高碘水。孕18日时，检测各组孕鼠甲状腺激素和促甲状腺激素水平；仔鼠出生后，取出生24 h 内仔鼠的颅骨成骨细胞进行培养，观察成骨细胞碱性磷酸酶活性和细胞增殖能力。结果碘缺乏组甲状腺激素含量较对照Ⅰ组、对照Ⅱ组和碘过量组明显降低（均 P ＜0．05），促甲状腺激素含量则明显升高（均 P ＜0．05）；碘过量组甲状腺激素含量与对照Ⅰ组和对照Ⅱ组间差别没有统计学意义（均 P ＞0．05），但促甲状腺激素含量则明显升高（均 P ＜0．05）。碘缺乏组成骨细胞碱性磷酸酶活性和细胞增殖能力较对照Ⅰ组、对照Ⅱ组和碘过量组明显下降（均 P ＜0．05）。结论大鼠孕期碘营养状态不足，可导致新生仔鼠成骨细胞活性和增殖能力下降。%Objective To study the effects ofiodine onosteoblasts in pup rats.Methods Female Wistar rats(n =20)after pregnan-cy were randomly divided into four groups:control group Ⅰ,control group Ⅱ,iodine deficient group,iodine overdose group.The control group Ⅰ was fed with a normal diet and drank deionized water,while the control group Ⅱ was fed with an iodine-deficient diet and drank deionized water with adequate iodine during the experiment.The iodine deficient group was fed with an iodine-deficient diet and drank deionized water during the experiment,while the iodine overdose group was fed with a normal diet and drank deionized water with high iodine.On gestational day 18,the levels of thyroid hormones and thyroid stimulating hormone were tested in pregnant rats.On post-natal day 0,alkalinephosphatase activities and proliferation of osteoblasts were observed in pup rats.Results The levels of thyroid hor-mones in iodine deficient group were significant lower than the controls(all P <0.05),while the levels of thyroid stimulating hormone increased significantly(all P <0.05).There were no significant differences in the levels of thyroid hormones between the iodine over-dose group and the controls(all P >0.05).However,a significant up-regulation of thyroid stimulating hormone was observed in the io-dine overdose group compared to the controls(all P <0.05).The alkalinephosphatase activities and proliferation of osteoblasts were sig-nificantly down-regulated in iodine deficient group compared to other groups(all P <0.05 ).Conclusions Iodine deficiency in preg-nant rats led to down-regulation of alkalinephosphatase activities and proliferation of osteoblasts inpup rats.