Ⅱ型糖尿病合并牙周病患者龈沟液中细胞因子／趋化因子的表达水平

摘要

Objective To explore the expression levels of cyto / chemokines in the gingival crevicular fluid (GCF)of type Ⅱ diabet-ic mellitus subjects with chronic periodontitis compared with those with simple periodontitis. Methods Fifty-two type Ⅱ diabetic mel-litus subjects and forty non-diabetic mellitus subjects with chronic periodontitis were enrolled in this study. The levels of 14 cyto / chemo-kines were measured in the GCF of healthy and diseased sites of the diabetic and non-diabetic mellitus subjects with chronic periodonti-tis using Luminex FLEXMAP3D plate reader and Human Cytokine / Chemokine kits. Results The concentrations of eotaxin,macro-phage inflammatory protein-1α,granulocyte-macrophage colony-stimulating factor,interleukin (IL)-6,tumour necrosis factor-α and IL-12 were higher at healthy and diseased sites of diabetic mellitus than non-diabetic mellitus subjects. Conclusions Type Ⅱ diabetes mellitus modulated the levels of several cyto / chemokines at both healthy and diseased periodontal sites. It may be a facilitating factor to periodontal disease.%目的：探讨Ⅱ型糖尿病合并牙周病患者与单纯牙周病患者龈沟液（gingival crevicular fluid，GCF）中细胞因子／趋化因子的表达水平。方法选取伴Ⅱ型糖尿病的牙周病患者52例，单纯牙周病患者40例，用Luminex FLEXMAP3D 仪和Human Cytokine ／ Chemokine 试剂盒检测GCF 中14种细胞因子／趋化因子的表达水平。结果牙周病部位：嗜酸性粒细胞趋化因子、巨噬细胞炎症蛋白－1α、粒细胞－巨噬细胞集落刺激、白介素－6、肿瘤坏死因子－α和白介素－12的浓度，糖尿病组受试者高于非糖尿病组受试者（ P ＜0．0035）。结论糖尿病可影响牙周病部位细胞因子／趋化因子的表达，糖尿病可能是牙周病的促进因素。