转录因子Brn3 a及胰岛因子1在三个时期中三叉神经节的表达情况

摘要

目的：探讨转录因子Brn3a及胰岛因子1(Islet1,Isl-1)转录因子在生长发育的三个时期中三叉神经节中的表达情况。方法取发育不同时期的小鼠胚胎头部第13.5天、15.5天、17.5天,各个时期为相互对照。用免疫荧光的方法标记相关蛋白,观察比较不同时期的Brn3a及Isl-1在三叉神经节的表达情况。结果三个时期中Brn3a及Isl-1在三叉神经节均有共表达,在生长发育早期Isl-1没有明显增殖,在生长发育晚期Brn3a没有明显凋亡。结论在发育后期Brn3a及Isl-1标记的神经元,依旧共表达于神经元中,且随生长发育,标记Brn3a及Isl-1的神经元逐渐减少。Brn3a及Isl-1标记的神经元,在生长发育过程中分化为亚型神经元,对上下游转录因子起调控作用。%Objective To investigate the expression of transcription factor Brn3a and Isl-1 in trigeminal ganglion during the three pe-riods of growth and development. Methods Mouse brains at 13. 5, 15. 5 and 17. 5 days after being born were used for contrast. The immunofluorescence technique was applied to investigate the expression patterns of Brn3 and Isl-1 in trigeminal ganglion between differ-ent periods. Results In all three periods, Brn3a and Isl-1 were co-expressed in the trigeminal ganglia. In the early stage of growth and development, Isl-1 showed no significant proliferation, and in the late stage of growth and development, Brn3a showed no signifi-cant apoptosis. Conclusion In the late stage of development, Brn3a and Isl-1 marked neurons were still expressed in the neurons, and with the growth and development, the marked Brn3a and Isl-1 neurons gradually decreased. Besides, during the process of growth and development, Brn3a and Isl-1 marked neurons differentiated into subtypes of neurons, which regulated the upstream and down-stream transcription factors.