种植体周龈沟液IL-17 A和IL-35水平检测及意义

摘要

Objective To analyze the similarities and differences of the expression of interleukin( IL)-17A and IL-35 between the group of peri-implantitis and peri-implant healthy patients, and to explore the mutual relationship and effect of IL-17A and IL-35 on the development and progress in peri-implantitis. Methods Peri-implant sulcular fluid( PISF) was collected from 20 subjects with healthy implants, and 20 with implantitis. The latter was made as comparison group after the implantitis was treated. The concentrations of IL-17A and IL-35 were determined by enzyme-linked immunosorbent assay method. Bleeding on probing(BOP), modified sulcus bleeding index( mSBI) and gingival index( GI) of the healthy implant group, the peri-implantitis group and the treated group were recorded and analyzed statistically. Results The concentrations of IL-17A and IL-35 in the peri-implantitis group were significantly higher than those in the healthy implant group (P<0. 05). IL-17A was slightly lower after treatment (P<0. 05), and IL-35 was slightly higher af-ter treatment (P<0. 05). The concentrations of IL-17A and IL-35 were negatively correlated in the peri-implantitis group. Conclusions The expressions of IL-35 and IL-17A are are closely related with the occurrence and development of peri-implantitis. IL-17A and IL-35 are potential molecular biological markers that may help assist diagnosis and treatment of implantitis.%目的：分析种植体组不同时期龈沟液(peri-implant sulcular fluid,PISF)中(interleukin,IL)-17A和IL-35水平改变,探讨其在种植体周围炎或黏膜炎发展过程中的作用和相互关系。方法收集2008—2013年在中山大学孙逸仙纪念医院口腔科行种植修复患者共40例为研究对象,分为种植体健康组20例和种植体炎症组20例,将种植体炎症组经过治疗后作为对照组。在种植体中有单冠修复、联冠修复。采集不同患者龈沟液并定量,对标本采用双抗体夹心酶联免疫吸附方法( Enzyme Linked Immunosorbent Assay,ELISA )测定各组龈沟液中 IL-17A和IL-35表达水平。并分别记录健康组、种植体炎症组、治疗后种植体炎症组探诊出血指数,改良出血指数,牙龈指数,并行统计学分析。结果种植体炎症组IL-17A,IL-35水平比健康组IL-17A,IL-35水平高(P<0.05),炎症组IL-17A水平治疗前比治疗后高(P<0.05),炎症组IL-35水平治疗后比治疗前升高(P<0.05),Pearson相关检验示,IL-35和IL-17A表达成负相关关系(P<0.05)。结论 IL-35和IL-17A的表达与种植体黏膜炎或者周围炎的发生发展密切,IL-17A和IL-35是辅助种植体周围炎诊治和评估抗炎疗效的潜在分子生物学指标。