新型低聚合收缩树脂材料的细胞毒性评价研究

摘要

目的 评价一种新型低聚合收缩树脂基质材料对L929小鼠成纤维细胞的细胞毒性.方法 将本课题组设计合成的具有低聚合收缩性能的树脂基质间三氟甲基苯基型丙烯酸酯3F-PAc与传统临床常用树脂基质Bis-GMA作比较,分为3组,分别为Bis-GMA组、3F-PAc 1组和3F-PAc 0组,3F-PAc 0组不添加稀释剂TEGDMA.制备3组材料浸提液,倍比稀释为4种浓度100%、50%、25%、12.5%;采用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法检测小鼠成纤维细胞L929与各稀释液共培养1、3、5 d后的细胞相对增殖率并与阴性对照组作比较,评价细胞毒性等级.结果 除培养3 d和5 d时Bis-GMA和3F-PAc 1组的100%浸液毒性等级为2级外,其余各实验组浸提液细胞毒性均为0~1级.3F-PAc 0组浸提液吸光度值大于3F-PAc 1组与Bis-GMA组,其差异具有统计学意义(P<0.05),3F-PAc 1组与Bis-GMA组之间差异不显著(P>0.05).结论 新型3F-PAc树脂基质材料无细胞毒性,具有较好的生物安全性;稀释剂TEGDMA可能是影响树脂材料细胞毒性的部分原因.%Objective To evaluate the cytotoxicity of a new type of low polymerization shrinkage resin matrix material on L929 mouse fibroblast cells.Methods The three fluorine meta-position methyl phenyl acrylate 3F-PAc was synthesized by our experimental group,which is a low polymerization shrinkage resin matrix.Then it was compared with the traditional clinical commonly used resin matrix Bis-GMA,and the experiment group was divided into three groups,Bis-GMA,3F-PAc 1 and 3F-PAc 0 group,among which 3F-PAc 0 group did not add the TEGDMA diluent agent.The immersed solution of the three groups of material was diluted to four grades 100%,50%,25% and 12.5%;the methyl thiazolyl tetrazolium(MTT) test was used to detect the relative cell proliferation rate of the mouse fibroblast cell line L929 co-cultured with the different solutions for 1,3,5 days.Compared with the negative control group,the cell toxicity grade was evaluated.Results All the cytotoxicity of the experimental group extracts was grade 0~1,except that the 100% extract of Bis-GMA and 3F-PAc 1 group were 2.The absorbance value (D) of 3F-PAc 0 groups was significantly higher than that of 3F-PAc 1 group and Bis-GMA group (P<0.05),and the difference was statistically significant.However,the difference between 3F-PAc 1 group and Bis-GMA group was not significant (P>0.05).Conclusions The new 3F-PAc resin matrix material has no cytotoxicity but has comparatively good biosecurity.The TEGDMA diluent agent may be a part reason of the cytotoxicity of the resin material.