紫外-可见光谱法研究细胞色素C活性中心金属离子与硫酸铜的相互作用

摘要

应用紫外-可见吸收光谱法研究细胞色素C（Fecyt-C）活性中心铁离子在pH 7.1的磷酸缓冲液中与无机CuSO4的直接相互作用。结果发现：CuSO4中的Cu(Ⅱ)与Fecyt-C中的Fe(Ⅱ)有交换作用，形成部分的Cucyt-C，并且随着外加CuSO4量的增加，这种相互作用增强，首次证实了细胞色素C这种直接相互作用的存在。%The direct interaction between the metal ion in the active center of cytochrome C (Fecyt-C) and inorganic copper sulfate in pH 7.1 phosphate has been studied by UV spectrometry.As a result,part of the metal ion Fe(Ⅱ) in Fecyt-C was replaced by Cu(Ⅱ) and the corresponding cytochrome C derivatives Cucyt-C were produced,in addition,the interaction was increased with amount of copper sulfate added.The direct interaction between the CuSO4 and metal ion of cytochrome C was verified for the first time.