落叶松树皮原花青素生产过程的红外光谱分析

摘要

为了评价现有的落叶松树皮原花青素中试生产过程,了解生产过程中各工艺流程对产品组分含量的影响,将各工艺处理后所得样品进行了一维红外光谱(fourier transform infrared spectroscopy,FTIR)、二阶导数光谱(second derivative infrared spectroscopy,SD-IR)及二维相关光谱(two-dimensional correlation in-frared spectroscopy,2D-IR)三级鉴定研究.实验结果表明,一维红外光谱中各样品光谱峰形较为相似,一些物质的特征峰消失或者偏移反映出样品组分含量的变化.随着生产工艺的进行,样品纯度、结构越来越接近原花青素标准品,但是仍含有少量其他物质.通过二阶导数谱进行分析,验证了一维光谱分析的结果,而且随着进一步提高谱图分辨率,将一维谱图中没有显现出来的差异直观的表现出来.在波数为830~1310 cm-1的二维相关光谱中,各过程样品自动峰出现的位置、个数以及强度有了更为显著的差异.通过红外三级光谱鉴定可以得出:随着生产过程的进行,样品中所含原花青素纯度越来越高,最终得到的样品结构与原花青素标准品极为相似,但是产品中仍然含有少量杂质.%In order to evaluate existing producing process of larixgmelinii bark proanthocyanidins ,understand the changes of the component content in each step ,we used Trilevel infrared spectroscopic to identify ,which employed the Fourier transform infrared spectroscopy(FTIR) ,the second derivative infrared spectroscopy(SD-IR) and two-dimensional correlation infrared spectroscopy .The results indicated that the FTIR spectrograms of the product of each process in shape was very similar .We found that some substances characteristic peak disappeared or offseted ,reflecting the changes of component content of samples .With the improvement of production process ,the purity and structure of samples have been more and more near to the standard sample ,but still contained some other substance .The second derivative infrared spectroscopy not only tested and verified the analysis results of FTIR ,but also improved the resolution ratio which was helpful to show differences that were not manifested in FT-IR .Within the scope of the 830 to 1310 cm-1 ,2D-IR has more significant differences in positions ,strength and numbers of automatically peaks .Therefore ,we could conclude from the Tri-level infrared spectroscopic identification that the purity in proanthocyanidins is higher and higher with the improvement of production process .And the structures of samples we get finally are similar to that of standard sample ,but still contains a small number of impurities.