分别采用超声、回流、浸泡法分离提取万寿菊中的叶黄素，再将其皂化提纯后进行液相测定，选出最佳分离提取方法。采用ODS C18填料色谱柱（4.6×250mm ，5μm ），柱温30℃，流速1mL/min ，进样10μL ，时间：22min ，流动相A （乙腈∶甲醇＝9∶1）∶B （乙酸乙酯）。样品采用梯度洗脱，0～5min ，B 10％；5～15min ，B 15％；15～20min ，B 0％。检测波长450nm。由叶黄素得率可知，浸泡提取法可作为提取万寿菊颗粒中叶黄素的有效实验方法，而且浸泡16h万寿菊中的叶黄素得率最多。叶黄素的质量浓度与峰面积分别在0.1～0.6mg/mL （ r＝0.9997）呈良好线性。通过标准品和样品的液相色谱图比较，确定皂化后所得的样品为叶黄素。证明此实验为最佳叶黄素的提取皂化实验。%Ultrasound ,reflux and soaking methods were used for separation of lutein marigold extract and then purified by liquid saponification its determination .The best method of separation and extraction was chosen.ODS C18 column using filler(4.6 × 250mm ,5μm) ,Column temper-ature at 30℃ ,flow at 1mL/min ,sample 10μL ,time:22min ,mobile phase A (acetonitrile∶methanol=9∶1 )∶B (ethyl acetate ) .Samples using a gradient elution ,0~5min when B Mobile phase at 10% ,5~15min when B Mobile phase was set at 15% ,15~20min when B Mobile phase was 0% ,the detection wavelength was at 450nm .Soaked by the lutein extraction yield can be seen as the extraction of lutein marigold particles effective experimental methods and soak 16h marigold lutein yield most .Lutein concentration and peak area ,respectively 0.1~0.6mg/mL( r=0.999 7 ) showed good linearity .Standards and samples by liquid chromatogram determined by comparing the sample obtained after saponifica-tion of lutein .This experiment proved the best lutein extraction saponification experiments .
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