木豆GDSL脂肪酶基因的克隆及表达分析

摘要

[Objective] The aim of the present paper is to discuss the adverse stress resistance mechanism of GDSL lipases.[Method] The GDSL lipase of C.cajan is cloned by RACE technology based on the preliminary study and then the expression characteristics are detected by fluorogenic quantitative PCR.[Result] An obtained GDSL lipase of C.cajan is named as CcGDSL1 and its total length of open reading frame is 1107 bp with 369 amino acids.The homology analysis shows that the amino acid sequence of CcGDSL1 has a high similarity with GDSL lipases of other legume plants.CcGDSL1 can express in leaf,stem and root but the expression quantity in leaf and stem is higher than in root significantly.Drought stress can increase the expression quantity of CcGDSL1 and the expression quantity of CcGDSL1 presents an expression pattern of first declining,then rising and finally stable with sustained drought.[Conclusion] CcGDSL1 may participate in stress reaction of C.cajan under drought stress.%[目的]为探明GDSL家族脂肪酶的抗逆机理,[方法]在前期研究基础上,利用RACE技术克隆木豆GDSL脂肪酶基因,并通过荧光定量PCR技术检测其表达特性.[结果]获得1条木豆GDSL脂肪酶基因,命名为CcGDSL1,该基因含开放阅读框全长1107 bp,编码369个氨基酸;经同源性分析,CcGDSL1编码的氨基酸序列与豆科植物中GDSL脂肪酶具有较高的相似性.该基因在叶、茎和根中均表达,且叶和茎中表达量显著高于根;干旱胁迫能提高CcGDSL1的表达量,随干旱的持续表现出先降后升随后趋于稳定的表达模式.[结论]推测CcGDSL1基因参与木豆应激干旱胁迫.