灯盏花内生真菌分离及其产黄酮内生真菌的初步研究

摘要

[Objective] In order to screen endophytic fungi which can produce flavonoids,the endophytic fungi in roots,stems,leaves and flowers of Erigeron breviscapus were isolated and identified.[Method] Endophytic fungi were isolated by using pure culture and selected produced flavonoids substances based on morphology and specific color reaction.[Result]40 endophytic fungi were isolated from Erigeron breviscapus and identified to fourteen genera based on morphology and screened seven strains produced flavonoids substances by means of specific color reaction.Seven strains produced flavonoids substances were identified as Alternaria sp.,Fusarium sp.,and Sirosporium sp.The highest quality concentration of flavonoids substances from endophytic fungi roots was 2.625 mg/L.[Conclusion] It has shown that quantity,species and distributing of the endophytie fungi varied in different parts of Erigeron breviscapus,and the concentration of flavonoids substances from endophytic fungi roots was the highest.%[目的]本研究对昆明市寻甸县人工栽培的大田灯盏花的根、茎、叶、花的内生真菌进行分离鉴定,旨在筛选产黄酮的内生真菌菌株.[方法]采用纯培养方法分离植物内生真菌,利用形态鉴定及显色反应筛选产黄酮内生真菌.[结果]共获得内生真菌40株,经显微形态观察40株内生真菌初步分类鉴定为14个属.通过黄酮类物质特异颜色反应,筛选到7株内生真菌产黄酮类物质,这7株内生真菌经初步鉴定为交链孢属(Alternaria sp.)、镰孢霉属(Fusarium sp.)、痕格孢属(Sirosporium sp.),根中内生菌产黄酮最高质量浓度为2.625 mg/L.[结论]灯盏花不同部位内生真菌的数量、分布和种群存有差异性,从根部分离到的内生真菌产黄酮类质量浓度最高.