血蓝蛋白在杂色鲍各组织中的分布研究

摘要

利用 Western blotting 方法对血蓝蛋白（hemocyanin）抗体浓度进行优化，进而运用免疫组化方法对杂色鲍（Haliotis diversicolor）血蓝蛋白在肝胰脏、外套膜、鳃、消化道、腹足中的分布规律进行了研究。免疫组织学检测发现血蓝蛋白在杂色鲍各组织中均有分布，其中肝胰脏、外套膜和鳃中分布较多，消化道和腹足中相对较少。%The distribution of hemocyanin in different tissues of Haliotis diversicolor was studied by immunohistochemistry. The opti-mum concentration of primary antibody was evaluated by Western blotting. Hemocyanin was shown to distribute in every issue,with stronger intensity of hybridization signal in hepatopancreas,mantle and branchia than in alimentary canal and pleopod.