为了探讨不同海藻多糖抗氧化活性的差异,对琼枝(Betaphycus gelatinae)、石莼(Ulva lactuca)、匍枝马尾藻(Sargassum polycystum)、南方团扇藻(Padina australis)和棒叶蕨藻(Caulerpa sertularioides)的粗多糖的自由基清除及脂质过氧化抑制能力进行了研究.结果表明,5种海藻多糖的抗氧化活性存在明显的差异性,南方团扇藻和匍枝马尾藻多糖具有较强的还原力,对超氧阴离子和羟自由基均有较强的清除活性,其中南方团扇藻多糖对超氧阴离子清除活性较强[半抑制浓度(IC50)为(262.00±24.60) μg· mL-],明显高于匍枝马尾藻多糖[IC50=(458.00±18.70) μg·mL-1],对羟自由基的清除活性[IC50=(388.00 ±45.29) μg·mL-1]与匍枝马尾藻多糖相近[IC50 =(312.04±37.42)μg·mL-1],匍枝马尾藻多糖对DPPH的清除能力[IC50=(95.80 ±7.48) μg·mL-1]显著高于其他4种海藻多糖,而南方团扇藻多糖对DPPH的清除能力较差[IC50=(726.00 ±54.90)μg·mL-1].通过体外抗脂质过氧化作用研究发现,南方团扇藻多糖对肝细胞膜脂质氧化有较高的抑制能力[IC50=(283.67±44.14) μg·mL-1],并且对过氧化氢诱导的红细胞溶解有一定的保护能力[IC50=(335.50±22.47) μg·mL-1].%To discuss the difference in antioxidant activity among different algal polysaccharides,we analyzed the free radical-scavenging and anti-lipid peroxidation capacity of crude polysaccharides extracted from Betaphycus gelatinae,Ulva lactuca,Sargassum polycystum,Padina australis and Caulerpa sertularioides in vitro.The results indicate that five algal polysaccharides were significantly different in antioxidant activity.The polysaccharides of S.polycystum and P.australis showed strong reducing power and free radical-scavenging capacity for superoxide anion and hydroxyl radical.The scavenging capacity for superoxide anion of P.australis polysaccharide [IC50 =(262.00 ± 24.60) μg·mL-1] was significantly higher than that of S.polycystum [IC50 =(458.00 ± 18.70) μg·mL-1],and the scavenging capacity for hydroxyl radical of P.australis polysaccharide [IC50 =(388.00 ± 45.29) μg·mL-1] was similar with that of S.polycystum [IC50 =(312.04 ± 37.42) μg·mL-1].The scavenging capacity for DPPH of S.polycystum polysaccharide [IC50 =(95.80± 7.48) μg·mL-1] was significantly higher than the other algal polysaccharides,while the scavenging ability for DPPH of P.australis polysaccharide was not good enough [IC50 =(726.00 ± 54.90) μg·mL-1].According to the anti-lipid peroxidation analysis in vitro,P.australis polysaccharide had higher inhibitory capacity for liver cell membrane lipid peroxidation [IC50 =(283.67 ±44.14) μg·mL-1],and certain ability to protect red cell lysis which was induced by hydrogen peroxide [IC50 =(335.50 ± 22.47)μg·mL-1].
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