一株高效解磷细菌的筛选、鉴定及其溶磷能力的研究

摘要

Various phosphate-solubilizing bacterial strains were isolated from the long term planting of vegetable greenhouse rhizosphere in Huangpi district of wuhan city.After many times of screening and purification,a strain P1 with efficient phosphate-solubilizing capability was obtained.According to physiological and biochemical characteristics and 16S rDNA sequence,the strain of P1 was identified as belonging to Ensifer.The effect of different fermentation conditions on the phosphate-solubilizing capacity of P1 strain was studied.Under the optimal conditions in which the fermentation time was 7 days,initial pH was 8 and inoculum size was 2%,P1 dissolved 443.11 mg/L tricalcium phosphate.The study aslo found that the capacity of the P1 was closely negative correlated to the pH of medium.%从武汉市黄陂区长期种植的蔬菜大棚作物根际分离筛选出多株解磷细菌,经过多次筛选纯化获得一株性状稳定的高效解磷细菌P1.根据生理生化特征和16S rDNA序列分析,鉴定菌株P1为根瘤菌属(Ensifer).研究了不同发酵条件对P1菌株解磷能力的影响,确定了菌株P1的最佳培养条件为发酵时间7 d、初始pH值8、接种量2%,在该条件下菌株P1溶解磷酸三钙的量为443.11 mg/L.试验还发现菌株P1的溶磷量与培养液的pH值呈极显著负相关性.