Ⅱ型VLDLR上调VEGF、MMP2和MMP7的表达促乳腺癌细胞迁移(英文)

摘要

Objective: Very low density lipoprotein receptor (VLDLR) has been considered as a multiple function receptor due to binding numerous ligands, causing endocytosis and regulating cellular signaling. Our group previously reported that type Ⅱ VLDLR overexpression in breast cancer tissues. The purpose of this study is to characterize type Ⅱ VLDLR activities during cell migration using breast cancer cell lines. Methods: Western blotting was used to test protein expression. Cell migration was analyzed by Scratch wound assay. The mRNA expression was tested by realtime-PCR. Reporter assay was to test the transcription activity. Results: Scratch wound and Report assay indicated up-regulated VLDLR Ⅱ expression promotes cell migration via activating Wnt/β-catenin pathway. The target genes such as VEGF, MMP2 and MMP7 were upregulated in VLDLR Ⅱ overexpressed cells. On the contrary, cells treated with TFPI had an inhibition effect of cell migration response to down-regulation of VLDLR Ⅱ. Conclusion: Type Ⅱ VLDLR conferred a migration and invasion advantage by activating Wnt/β-catenin pathway, then up-regulating VEGF, MMP2 and MMP7 in breast cancer cells.