力竭运动后大鼠外周血mir-24与大脑皮质Bax表达运动性中枢疲劳

摘要

目的：探讨一次性力竭游泳运动后大鼠外周血mir-24和大脑皮质bax基因表达变化与运动性中枢疲劳的关系。方法：健康雄性Wista大鼠48只,随机分为对照组（C组）、一次性力竭运动组（E组）。力竭运动后即刻和24h取各组外周血和大脑皮质分别采用real-time PCR和TUNEL法等技术检测各组外周血mir-24和大脑皮质内bax基因表达情况和凋亡情况。结果：1次性力竭运动后即刻,大鼠外周血mir-24和大脑皮质内bax基因的表达均升高（P〈0.05）,24小时后均基本恢复到安静水平（P〉0.05）,这与TUNEL检测到凋亡情况一致。结论：凋亡可能是运动性疲劳发生的机制之一,外周血mir-24可能作为运动性疲劳的监测指标。%The purpose of this experiment is to study the relationships between the expression of mir-24 in peripheral blood and bax in cerebral cortex and sport central neural system fatigue after exhaustive swimming.Depending on results of real-time PCR and TUNEL,the present study suggests that the expression of mir-24 in peripheral blood may be a promising marker for sport central neural system fatigue.