目的 探讨周期蛋白(cyclin D1)在肺癌中的表达及对其生物学行为的影响.方法 采用免疫组织化学(SP)法,对120例肺癌及癌旁正常组织中cyclin D1进行检测.结果 cyclin D1蛋白阳性产物主要定位于胞核,呈棕黄色颗粒,少部分定位于胞质.本研究cyclin D1在癌旁正常组织中的阳性表达率为8.3%,肺癌中的阳性表达率为70.0%,差异有统计学意义(P<0.05);在不同组织学类型肺癌中的阳性表达率有所不同,非小细胞肺癌(NSCLC,包括鳞癌 、腺癌 、大细胞癌)与小细胞肺癌(SCLC)之间的比较差异有统计学意义(P<0.05);在不同分化程度 、有无淋巴结转移及TNM分期组间比较差异无统计学意义(P>0.05).结论 cyclin D1的表达可能是发生在NSCLC的早期事件.%Objective To explore the expression of cyclin D1 in lung cancer and its influence on biological behavior .Methods Using immunohistochemical method by S-P kit ,the expressions of cyclin D1 in cancer and normal tissue adjacent to cancer were examined in 120 cases of lung cancer .Results Cyclin D1 protein positive products ,which are tan-yellow particles ,are mainly located in the cell nucleus ,and some of them are positioned in the cytoplasm .The expressions of normal tissue adjacent to cancer with ones of cancer were compared ,the differ-ence is statistically significant(P< 0 .05) ;in different histologic types of lung cancer ,the positive expression rate is different ,and the difference between non-small cell lung cancer (squamous cell carcinoma ,adenocarcinoma ,large cell carcinoma) and small cell carcinoma is statistically significant (P < 0 .05) ;the relative differences among ex-pressions in different differentiation degree ,with or without lymph node metastasis and in TNM staging are not statistically significant (P > 0 .05) .Conclusion Expression of cyclin D1 may be an early event occurred in non-small cell lung cancer .
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