肿瘤坏死因子-α诱导蛋白8对卵巢癌细胞株的增殖迁移影响分析

摘要

目的 探讨肿瘤坏死因子-α诱导蛋白8(TNFAIP8)对卵巢癌细胞增殖、迁移的影响.方法 收集自2013年6月至2015年6月在我医院接受诊治的卵巢癌患者60例作为研究对象,免疫组织化学法检测卵巢癌组织中TNFAIP8蛋白的表达情况,并分析其与临床病理参数的关系.人卵巢癌SKOV3细胞分别转染pCMV6-TNFAIP8 质粒(A组)以及空白质粒pCMV6(B组),蛋白质印迹法检测转染效率,四甲基偶氮唑蓝(MTT)法检测细胞增殖活性,细胞划痕实验检测细胞迁移能力.结果 卵巢癌组织阳性表达率(82%)显著高于癌旁组织的阳性表达率(15%),且与卵巢癌的分级显著相关. 转染质粒后,A组卵巢癌 SKOV3细胞与B组相比, TNFAIP8蛋白表达显著上升,且A组卵巢癌 SKOV3细胞的增殖能力以及迁移率显著高于B组.结论 TNFAIP8在卵巢癌组织中表达显著升高,这种高表达可以促进卵巢癌细胞的增殖以及迁移,这显示TNFAIP8可以作为一种新的分子靶点用于卵巢癌诊疗.%Objective To study the effects of TNFAIP8 on proliferation and migration of ovarian cancer cells.Methods Sixty patients with ovarian cancer admitted in our hospital between June 2013 and June 2015 were enrolled in this study.Immunohistochemistry was applied to detect the expression of TNFAIP8, and the relationship between TNFAIP8 and the clinical pathological features was analyzed.Ovarian cancer SKOV3 cells were transfected with pCMV6-TNFAIP8 plasmid (group A) and blank plasmid pCMV6 (group B).The transfection efficiency was detected by Western blotting, the proliferation was assayed by MTT method, the migration was assayed by wound-healing.Results The positive rate of ovarian cancer tissue (82%) was significantly higher than that in the adjacent tissues (15%), which was significantly correlated with the grade of ovarian cancer.After transfected with plasmid, the expression of TNFAIP8 in group A was significantly higher than that in group B, the proliferation and migration of ovarian cancer cells in group A were significantly higher than those in group B.Conclusion The expression of TNFAIP8 in ovarian cancer tissue was significantly increased, and this overexpression of TNFAIP8 could promote the proliferation and migration of ovarian cancer cells.The TNFAIP8 can be used as a new molecular target to evaluating the treatment and prognosis of ovarian cancer.