关节六经围刺法配合指（趾）端放血治疗糖尿病周围神经病变实验研究

摘要

目的：研究关节六经围刺法配合指(趾)端放血疗法对糖尿病大鼠坐骨神经传导速度及下肢微循环血流量的影响。方法用链脲佐菌素造模，分为模型组、弥可保组、针刺组、正常组，弥可保组按50g/kg 隔日注射甲钴胺注射液于下肢肌肉，左右腿交替注射；针刺组取双侧太溪、昆仑、解溪穴，每日针刺1次，配合大鼠足趾端放血，隔日1次；正常组和模型组隔日捆绑固定1次，每次30 min。各组均治疗8星期，分别于治疗前和治疗后两个时间点观察大鼠一般情况及测定各组坐骨神经传导速度，以及下肢微循环血流量。结果与正常组比较，各组大鼠坐骨神经传导速度均减慢(P＜0.01)；与模型组比较，各治疗组大鼠坐骨神经传导速度均有提高(P＜0.05)。结论关节六经围刺配合指(趾)端放血疗法能够提高糖尿病模型大鼠坐骨神经传导速度，改善下肢微循环。%Objective To investigate the effect of surround needling at articular six meridians plus finger (toe) tip bloodletting therapy on sciatic nerve conduction velocity and lower limb microcirculatory flow in diabetic rats. Method The model was made with streptozotocin. The rats were allocated to model, methycobal, acupuncture and normal groups. The methycobal group received a muscular injection of 50 g/kg in the left and right lower limbs alternately, every other day. The acupuncture group received acupuncture at points Taixi(KI3), Kunlun(BL60) and Jiexi(ST41), once daily plus toe tip bloodletting. The normal and model groups were bound 30 min once every other day. Every group was treated for eight weeks. General condition was observed, and sciatic nerve conduction velocity and lower limb microcirculatory flow were measured in the rats before and after treatment. Result Compared with the normal group, sciatic nerve conduction velocity decreased in the other groups of rats (P<0.01). Compared with the model group, sciatic nerve conduction velocity was higher in every treatment group of rats (P<0.05). Conclusion Surround needling at articular six meridians plus finger (toe) tip bloodletting therapy can increase sciatic nerve conduction velocity and improve lower limb microcirculation.