HPLC-MS同时测定泽泻中两种泽泻醇的含量

摘要

To improve the quality control of the Rhizoma Alismatis,a HPLC-MS method for determination of the contents of two alisols in Rhizoma Alismatis was established in this paper.Chromatographic conditions were as follows:Halo C18column (2.1 mmx100 mm,2.7 μm),the mobile phase was a mixture of 0.1％ formic acid solution-0.1％ formic acid in acetonitrile,gradient elution,the flow rate was 0.3 mL · min-1,the column temperature was 30 ℃.The conditions of MS included:ESI ion source,detection method with positive ions,mode by MRM,drying temperature was 350 ℃,the flow rate of drying gas was 9 L · min-1,pressure of nebulizer was 241.3 kPa,and the voltage of capillary was 4 000 V.The content of alisol B 23-acetate and alisol A 24-acetate in the samples was 11.4595 μg · g-1and 321.823 9 μg · g-1,respectively.The method is accurate and reproducible to the determination of two alisols in Rhizoma Alismatis.%为提高泽泻药材的质量控制水平,建立了泽泻中23-乙酰泽泻醇B和24-乙酰泽泻醇A的高效液相-质谱联用含量测定方法.色谱条件为Halo C18色谱柱(2.1 mm×100 mm,2.7 μm),体积分数0.1％甲酸水-0.1％甲酸乙腈为流动相,梯度洗脱,流速0.3 mL·min-1,柱温30℃;质谱条件为ESI离子源,正离子检测方式,MRM模式,干燥气温度350℃,干燥气流速9L·min-1,雾化器压力241.3 kPa,毛细管电压4000V.测得药材中23-乙酰泽泻醇B和24-乙酰泽泻醇A含量分别为11.459 5μg·g-1,321.823 9μg·g-1.该方法结果准确,重现性好,可用于测定泽泻中两种成分的含量.