Eps8在恶性血液肿瘤细胞株中的表达分析

摘要

Objective To observe the expression of Eps8 in human hematologic neoplasms cell lines. Methods Real time quantitative RT-PCR and Western blot assay were used to detect the expression of Eps8 mRNA and protein in 6 kinds of hematologic neoplasms cell lines. Results Compared with peripheral blood mononuclear cells ( PBMNCs), the expres sion of Eps8 mRNA in ARH-77 cell line increased ( 1. 278 6 ± 0. 188 0) -fold (P =0.008), and that in Raji cell line was (0.011 0 ± 0.001 0) -fold (P = 0.000). The expression of Eps8 protein was higher in KG1 a and ARH-77 cell lines, but the other 4 kinds of cell lines and PBMNCs had no expression of Eps8 protein. Conclusion It's the first time that we find Eps8 protein express highly in some hematologic neoplasms cell lines, which maybe provide a new trend on the targeted therapy of hematologic neoplasms.%目的 探讨表皮生长因子受体通路底物8(Eps8)在人类恶性血液肿瘤细胞株中的表达情况及其差异.方法 分别利用实时荧光定量RT-PCR法及Western blot法检测6种恶性血液肿瘤细胞株中Eps8 mRNA及蛋白的表达情况.结果 ARH-77细胞株中Eps8 mRNA为健康者外周血单个核细胞(PBMNCs)中的(1.278 6±0.188 0)倍(P=0.008),Raji细胞株中为PBMNCs中的(0.011 0±0.001 0)倍(P=0.000).EPS8蛋白在KG1a、ARH-77细胞株中高表达,在其他4种细胞株及健康正常人PBMNCs中均未见Eps8蛋白表达.结论 本研究首次发现了Eps8在部分恶性血液肿瘤细胞株中高表达,为恶性血液肿瘤治疗新靶点的筛选提供了理论依据.