Objective To study significance of miRNA-146a expression in patients with nasopharyngitis and nasopha-ryngeal carcinoma (NPC). Methods The expressions of miRNA-146a in nasopharyngeal biopsy tissues of the 13 chronic nasopharyngitis patients and 16 NPC patients were detected by SYBR Green quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. To compare difference of miR-146a expression in chronic nasopharyngitis and NPC and different clinicopathologic characteristics of 16 NPC cases. Results miRNA-146a levels in tissues of NPC were significantly higher than those of chronic nasopharyngitis (P <0.01). Also, they were higher in the lymphatic gland metastasis group than those in no lymphatic gland metastasis group (P <0.05). There was no statistical difference in different age groups and TNM clinical stage groups(P > 0. 05). Conclusions miRNA-146a is high-expressed in tissue of NPC, which may promote lymphatic metastasis.%目的 探讨miRNA-146a在鼻咽炎症和癌症病变组织中的表达及意义.方法 SYBR Green实时定量PCR方法定量检测miRNA-146a在13例鼻咽黏膜慢性炎症患者和16例鼻咽癌患者病变组织中的表达,比较miR-NA-146a表达在鼻咽炎症与癌症组织中以及鼻咽癌不同临床病理特征的组间差异.结果 鼻咽癌组织中miRNA-146a表达水平明显高于鼻咽黏膜慢性炎症组织(P ＜0.01)；miRNA-146a在鼻咽癌淋巴结转移组中的表达水平高于无淋巴结转移组(P＜ 0.05)；miRNA-146a表达水平在不同年龄及临床分期比较差异均无统计学意义(P均＞0.05).结论 人鼻咽癌组织中miRNA-146a呈高表达,可能促进鼻咽癌淋巴道转移.
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