阿尔茨海默病患者血清miR-9水平变化及意义

摘要

Objective To investigate the value of serum MicroRNA-9 ( miR-9 ) in the early diagnose of Alzheimer′s disease(AD).Methods Serum samples were obtained from 75 old AD patients(case group) whose minimum mental state examination(MMSE) scored 13.27 ±2.53 and 85 old normal controls(normal group) to detected miR-9 level by the meth-od of real-time quantitative reverse transcriptase PCR ( qRT-PCR) .The clinical diagnosis value was evaluated by receiver operating characteristic( ROC) result to analyze it′s sensitivity and specificity.Results Compared with normal group, ser-um MiR-9 level of case group decreased significantly (P=0.012).The sensitivity and specificity in the diagnosis of AD was 80.5%and 71.8%, respectively.MiR-9 was negatively correlated with MMSE score (r=-0.603, P<0.01).Con-clusions Serum miR-9 can be used as an early diagnose biomarker for AD.%目的：探讨微小RNA-9（miR-9）在阿尔茨海默病（AD）早期预警和诊断中的价值。方法采用荧光定量PCR法检测75例老年AD患者[简短精神状态量表（MMSE）评分（13．27±2．53）分]及85例正常老年人血清miR-9表达，通过ROC曲线分析其诊断AD的特异性及灵敏性。结果与对照组相比，miR-9表达水平显著下降（ P＝0．012），miR-9诊断AD的敏感度和特异度分别为80．5％和71．8％；miR-9水平变化与MMSE呈负相关性（r＝-0．603， P＜0．01）。结论血清miR-9可作为AD早期预警和诊断的生物学标记物。