蜂胶黄酮对炎症反应单核细胞中 NLRP3炎性小体活性的影响

摘要

Objective To explore the effects of propolis flavonoids on the activation of oxidized low-density lipoprotein (ox-LDL) induced nucleotide-binding oligomerization domain-like receptor containing protein 3 (NLRP3) inflammasome in mononuclear cells .Methods The monocytes THP1 were cultured and divided into five groups .The experimental groups 1, 2, 3 and the model group were treated with 100 μg/mL ox-LDL to produce mononuclear inflammatory reaction models . The monocytes of the experimental groups 1, 2 and 3 were treated with 5, 10 and 15μg/mL propolis flavonoids before ox-LDL, and the monocytes of the control group was treated by ox-LDL in the absence of propo1is flavonoids.After 24 h, the expression of NLRP3 was measured by Western blotting .The levels of IL-1βand IL-18 were measured by ELISA .Results The levels of NLRP3 protein in the control group , model group and the experimental groups 1, 2 and 3 were 0.26 ± 0.04, 1.12 ±0.15, 0.98 ±0.11, 0.65 ±0.07 and 0.52 ±0.06, respectively.The expression of NLRP3 protein was sig-nificantly increased in the model group as compared with that in the control group (all P<0.05).The expression of NL-RP3 protein in the experimental groups 2 and 3 was significantly decreased as compared with that of the model group ( all P<0.05).The levels of IL-1βand IL-18 was significantly higher in the model group as compared with those of the control group, and the levels of IL-1βand IL-18 in the experimental groups 2 and 3 was significantly decreased as compared with those of the model group (all P<0.05).Conclusion Propolis flavonoids may down-regulate the expression of NLRP3 protein in the ox-LDL induced inflammatory response of the mononuclear cells , decrease the levels of IL-1βand IL-18 and inhibit the activation of NLRP 3 inflammasome .%目的：观察蜂胶黄酮对氧化型低密度脂蛋白（ ox-LDL）诱导的炎症反应单核细胞中核苷酸结合寡聚化结构域样受体3（NLRP3）炎性小体活性的影响。方法培养单核细胞THP1并分为5个组。实验1、2、3组及模型组加入ox-LDL 100μg／mL制作单核细胞炎症反应模型；实验1、2、3组在加入ox-LDL前分别给予5、10、15μg／mL蜂胶黄酮处理1 h；对照组不添加药物。各组培养24 h后，采用Western blotting 法检测细胞中的NLRP3蛋白，采用ELISA法检测细胞培养液上清中的IL-1β、IL-18。结果对照组、模型组、实验1组、实验2组、实验3组细胞中NL-RP3蛋白相对表达量分别为0．26±0．04、1．12±0．15、0．98±0．11、0．65±0．07、0．52±0．06，模型组与对照组相比， P均＜0．05；实验2组、实验3组与模型组相比，P均＜0．05。模型组培养液上清中IL-1β、IL-18水平高于对照组，实验2组、实验3组培养液上清中IL-1β、IL-18水平低于模型组（P均＜0．05）。结论蜂胶黄酮可下调ox-LDL诱导的单核细胞炎症反应模型中NLRP3蛋白的表达，降低IL-1β、IL-18分泌水平，抑制NLRP3炎性小体的活性。