Anti-CD20与 IL-10联合应用对非肥胖型糖尿病小鼠脾脏免疫功能的影响

摘要

Objective To observe the influence of anti-CD20 monoclonal antibody ( Anti-CD20 ) combined with inter-leukin 10 ( IL-10) on the immune function of spleen in non-obese diabetic ( NOD) mouse.Methods Twenty-four NOD mice were randomly divided into four groups , 6 mice in each group:groups A, B, C and D, which were caudal venously injected with 250μg Anti-CD20 , 250μg Anti-CD20 +0.1 mL IL-10, 0.1 mL IL-10 and 0.1 mL normal saline at day 1, 8, 15 and 21, respectively.All mice were sacrificed 12 weeks after first injection and the spleens were immediately taken out . The number of CD4+T cells, CD3+T cells and the levels of IL-10 and IL-27 were detected by immunohistochemistry . Results Compared with group D, the number of CD+4 T cells, CD+3 T cells and the levels of IL-10 and IL-27 in the spleen tissues were increased in the groups A , B and C (all P<0.01).Compared with groups A and C , the number of CD4+T cells, CD+3 T cells and the levels of IL-10 and IL-27 in the spleen tissues were increased in the group B (all P<0.01). Conclusion The combined administration of Anti-CD20 and IL-10 increases the number of CD+4 T cells, CD+3 T cells and the levels of IL-10 and IL-27 in the spleen tissues , and thus it regulates the immune microenvironment and prevents the oc-currence of diabetes .%目的：观察抗CD20单克隆抗体（Anti-CD20）与白细胞介素10（IL-10）联合应用对非肥胖型糖尿病（NOD）小鼠脾脏免疫功能的影响。方法将24只NOD小鼠随机分为A、B、C、D组各6只，分别于第1、8、15、21天尾静脉注射Anti-CD20250μg、Anti-CD20250μg ＋IL-100．1 mL、IL-100．1 mL和生理盐水0．1 mL。首次用药后第12周断颈处死小鼠，立即取出脾脏，采用免疫组化法检测脾脏组织中的CD4＋、CD3＋T细胞及IL-27、IL-10。结果与D组比较，A、B、C组脾脏组织中CD4＋、CD3＋T细胞数量及IL-27、IL-10表达增加（ P均＜0．01）；与A、C组比较，B组脾脏组织中CD＋4、CD＋3 T细胞数量及IL-27、IL-10表达增加（P均＜0．01）。结论 Anti-CD20与IL-10联合应用可增加NOD小鼠脾脏组织中CD＋4、CD＋3 T细胞数量及IL-27、IL-10表达，从而调节机体免疫微环境，预防糖尿病的发生。