甜樱桃乙烯转录因子PaEIL1基因的克隆及序列分析

摘要

The fragment of PaElLl was isolated from mature sweet cherry fruit by RT - PCR using degenerate primers corresponding to the EIN3/EIL conserved region sequence. Both 5' and 3' end sequences of the cDNA were cloned using RACE technique. PaEILl was 2 636 bp in length, while the predicted open reading frame (ORF) of the cDNA was 1 806 bp and the deduced amino acid sequence of PaEILl consisted of 601 amino acids. The results of phylogenic analysis indicated that PaEILl could be classified into EIN3/EIL protein family.%根据植物乙烯转录因子EIL( EIN3- like)家族的氨基酸和核苷酸保守区序列设计简并引物,以甜樱桃(Prunus avium L.)果实的cDNA为模板,通过RT- PCR方法得到了PaEIL1部分序列.随后进一步通过RACE技术克隆得到了PaEIL1全长片段,其大小为2636 bp,包含一个1 806bp大小的完整开放阅读框(ORF)及部分非编码区序列,编码蛋白大小约为601个氨基酸.聚类分析结果表明,PaEIL1属于EIN3/EIL家族乙烯转录因子成员.