利用wx基因分子标记快速鉴定糯性小麦

摘要

为了加快培育糯性小麦新品种,联合运用wx-B1基因的STS-PCR标记及wx-A1、wx-D1基因的SSR标记对济麦22×Waxy杂交组合F3代群体的131个单株进行wx基因型鉴定.结果发现:在131株F3代分离群体材料中,单缺失wx-A1a或wx-B1a或wx-D1a基因的材料分别为69、29、35株,分别占52.7%、22.1%和26.7%;同时缺失wx-B1a和wx-D1a基因的材料为10株,占7.6%;同时缺失wx-A1a和wx-B1a基因的材料为13株,占9.9%;未发现同时缺失wx-A1a和wx-D1a基因单株及wx-A1a、wx-B1a和wx-D1a基因全部缺失的单株.%In order to accelerate breeding new waxy wheat varieties , 131 F3 segregation plants of Jimai22 × Waxy were used for identification of wx genotypes with STS and SSR markers. The number of plants lacked of wx-A1α,wx-B1α or wx -D1α were 69,29 and 35 respectively;10 plants lacked both wx -B1αand wx -D1α; 13 plants lacked both wx - A1α and wx -B1α. No plants lacked both wx -A1α and wx -D1α and all of the three wx genes.