不同小麦品种的潮霉素耐受性研究

摘要

Hygromycin is one of the most popular selection agents applied to genetic transformation system. In order to study the hygromycin tolerance of different wheat varieties, 0, 25, 50, 75,100,125 and 150 mg/L hygromycin were added to the medium for immature embryo rescue and tolerance test of five wheat varieties (K35,065657,Yangmai 158 ,Bobwhite and Jiyin 1 ) with high embryo regeneration capacity. The results indicated that the optimal hygromycin concentration for screening the positive transformant derived from K35 and 065657 was 125 mg/L, while that for Yangmai 158, Bobwhite and Jiyin 1 was 100 mg/L.%潮霉素是基因转化过程中常用的筛选剂,为了探索不同小麦品种对潮霉索的抗性,本研究设置0、25、50、75、100、125、150 mg/L不同的潮霉素浓度,对K35、065657、扬麦158、Bobwhite和济引1号5个幼胚再生率高的小麦品种进行幼胚拯救并测试其对潮霉索的耐性.结果表明:K35和065657幼胚的最适筛选浓度是125 mg/L,扬麦158、Bobwhite和济引1号幼胚的最适筛选浓度是100 ms/L.