防风愈伤组织诱导及植株再生体系研究

摘要

以防风为材料,研究不同外植体、激素组合、培养基对愈伤组织诱导及植株再生的影响.结果表明,茎段是防风组织培养较为理想的外植体材料.诱导愈伤组织的最佳培养基为MS +1.5 mg/L2,4-D+0.5 mg/L KT+ 1.0 mg/L 6 - BA,最高出愈率为96.7％；诱导不定芽的最佳培养基为MS+ 1.0 mg/L 6 - BA+0.5 mg/L NAA,最高诱导率为75％；诱导根的最佳培养基为1/2MS +0.5 mg/L NAA +0.1 mg/L 6 - BA,生根率达65％；组培苗的移栽成活率达80％.%Different explants of Saposhnikovia divaricata were used to study the influence of different hormone combinations and media on callus induction and plant regeneration. The results showed that stem segments were the optimal explants; the optimal medium for callus induction was MS + 1.5 mg/L 2,4 - D +0. 5 mg/L KT + 1.0 mg/L 6 - BA, and on it, the callus forming rate could reach to 96. 7% ; the optimal medium for induction of adventitious buds was MS + 1.0 mg/L 6 - BA + 0. 5 mg/L NAA, and the highest induction rate was 72%. 1/2MS + 0. 5 mg/L NAA + 0. 1 mg/L 6 - BA was the best for rooting, and on which, the rooting rate could reach to 65%. The transplanting survival rate of culture seedlings could reach to 80%.