随着高通量测序技术的不断发展和测序成本不断降低,高通量测序近几年在现代农业研究领域中得到了充分应用,为新品种选育和品质改良带来了新的科研方法和解决方案,加快了新品种的育种进程.高通量测序技术的主要应用方向包括对农作物和栽培品种进行全基因组从头测序和深度重测序、遗传差异分析、分子标记开发、遗传连锁分析、表观遗传分析和转录组分析等.本文系统阐述了近几年高通量测序技术在农业研究中的应用进展,展示高通量测序在现代农业研究领域的广泛应用前景.%In recent years, with the development and sequencing costs reducing, the high - throughput sequencing technology gets full uses in modern agricultural research. It provided new method for breeding new varieties and quality improvement, and speeded up the breeding process. The main application of high -throughput sequencing technology including whole genome de novo sequencing and depth re - sequencing, genetic difference analysis, development of molecular markers, genetic linkage analysis, epigenetic analysis and transcriptome analysis. In this review, the utilization of high - throughput sequencing technology in agricultural research during the past several years was outlined, and its broad application prospect in modern agricultural biotechnology was showed, too.
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