花生果针离体培养研究

摘要

Using Zhonghua 8 as experimental material,the effects of GA,6 -BA and auxin (IAA and NAA)on peg culture in vitro of peanut were studied.The results indicated that the optimum medium con-tained inorganic salts of MS,organic constituents of B5,300 mg/L casein hydrolysate and 6% sucrose and supplemented with 1.0 mg/L GA,0.5 mg/L IAA and 0.01 mg/L 6 -BA.Under this condition,the peanut ovary enlargement rate was 72%.%以中华8号花生品种为试材，研究了 GA、6－BA 和生长素（IAA 和 NAA）对花生果针离体培养的影响。结果表明，花生果针离体培养的最佳培养基配方为 MS 培养基的无机盐成分、B5培养基的有机成分、300 mg／L 水解酪蛋白、6％蔗糖、1．0 mg／L GA、0．5 mg／L IAA 和0．01 mg／L 6－BA，在此条件下，花生子房膨大率为72％。