24个石榴品种的抗寒性评价

摘要

结合低温胁迫过程生理生化指标的变化,以一年生休眠枝条为试材对24个石榴品种进行抗寒性评价.采用人工模拟低温(室温、0、-4、-8、-12、-16、-20℃)进行低温处理,对其相对电导率(REC)、半致死温度(LT50)、脯氨酸(Pro)和丙二醛(MDA)含量进行测定,并采用隶属函数法对24个石榴品种的抗寒性进行综合评价.结果表明,随着胁迫温度的降低,24个石榴品种一年生枝条的相对电导率、脯氨酸含量、丙二醛含量变化总体呈上升趋势.通过半致死温度得出,‘峄城抗寒1号’的半致死温度最低,为-14.32℃,最抗寒;‘突尼斯软籽石榴’的半致死温度最高,为-7.95℃,最不抗寒.通过隶属函数法进行抗寒性综合评价,结果显示24个石榴品种中,有8个品种抗寒等级为Ⅱ级(抗)、15个品种抗寒等级为Ⅲ级(中抗)、1个品种抗寒等级为Ⅳ级(低抗).%The cold resistance of 24 pomegranate cultivars was evaluated by the changes of physiological and biochemical indexes under low temperature stress with one-year-old dormant shoots as materials.Adopting simulated low temperature (room temperature,0,-4,-8,-12,-16,-20℃) for low temperature treatment,the relative conductivity (REC),semilethal temperature (LT50) and proline (Pro) and malondialdehyde (MDA) contents were determined and the cold resistance of 24 pomegranate cultivars was evaluated comprehensively by membership function method.The results showed that the REC,Pro and MDA contents of one-year-old shoots of 24 pomegranate cultivars showed an increasing trend with the decreasing of stress temperature.The LT50 of Yicheng Kanghan 1 was the lowest,which was-14.32℃ and showed the best cold resistance;the LT50 of Tunisi soft-seed pomegranate was the highest,which was-7.95 ℃ and showed the worst cold resistance.Through the membership function method for comprehensive evaluation of cold-resistance,we could concluded that 8 cultivars were classified into the grade Ⅱ with cold resistance,15 cultivars were classified into the grade Ⅲ with moderate resistance and 1 cultivar belonged to the grade Ⅳ with low resistance.