外源性 Apelin-13对急性心肌梗死大鼠心肌细胞凋亡的影响研究∗

摘要

目的：研究外源性 Apelin-13对急性心肌梗死大鼠心肌细胞保护作用。方法：通过结扎左冠状动脉前降支方法建立急性心肌梗死大鼠模型,存活大鼠分为心梗组(AMI 组)及 Apelin-13治疗组,另设假手术组(Sham 组)及假手术 Apelin-13治疗组(Sham+Apelin-13组)。治疗14d 后,取心脏组织,TTC/Evans Blue 染色检测心肌梗死面积；HE 染色检测心脏组织病理变化；TUNEL法检测心肌组织细胞凋亡阳性率；Western blot 法检测凋亡蛋白 Bcl-2、Bax、Cleaved-Caspase-3的表达。结果：与急性心肌梗死模型组相比,Apelin-13治疗后,大鼠心脏组织梗死率显著减小,心肌组织炎性细胞富集减少,凋亡心肌细胞数减少,促凋亡蛋白 Bax、cleaved-Caspase-3表达减少,抗凋亡蛋白 Bcl-2表达增多。结论：Apelin-13可以有效缓解缺血引起的急性心肌梗死和心肌细胞凋亡,可以作为临床治疗急性心肌梗死的备选药物。%Objective:To study the protection effect of Apelin-13 on myocardial cell of acute myocardial infarction in rats.Methods:The acute myocardial infarction methods (AMI)model was developed by ligation of the left anterior descending coronary artery.The surviving rats were divided into AMI group (AMI)and Apelin-13 trea-ted group (Apelin-13),and the groups of sham operation and Apelin-13 treated sham operation were also set up(n=8).After treatment for 14d,the rat hearts were taken,then detected the myocardial infarction area by TTC&Evans Blue staining and the pathological changes of cardiac tissue through HE staining.Furthermore,TUNEL method was used to detect the apoptotic myocardial cells,and the expression of the apoptotic protein Bcl-2,Bax and cleaved-Caspase-3 were detected by Western blotting.Results:Compared with the AMI group,after Apelin-13 treatment, the relative area of infarction myocardium in rats were significantly reduced,the accumulation of inflammatory cell in myocardial tissue and the number of apoptotic myocardial cells were decreased,the expression of pro-apoptotic pro-tein Bax and cleaved-Caspase-3 were increased and the expression of anti-apoptotic protein Bcl-2 was decreased.Con-clusion:Apelin-13 could effectively alleviate the acute myocardial infarction and myocardial cell apoptosis induced by myocardial ischemia,and Apelin-13 can be used as a drug for clinical treatment of acute myocardial infarction.