Objective:To investigate change of circulating microparticles (MPs) in pulmonary hyperten‐sion rats and its relationship with pulmonary hypertension. Methods:48 healthy male Wistar rats were randomly di‐vided into three groups. All rats except those in control group were injected intraperitoneally (ip) with a single dose (50 mg/kg) of MCT to induce PH. Right ventricular systolic pressure (RVSP) was measured at 3 and 5 weeks be‐fore extracting rat blood from abdominal aortic for MPs detecting. Rat pulmonary artery (n= 6) were stimulated with the extracted MPs, changes of endothelial nitric oxide synthase (eNOS), elastin were detected. Results :Plas‐ma MPs level in rats increased gradually with PH disease progression (M5 > M3 >C);MPs down‐regulate eNOS, but up‐regulate elastin expression in rat pulmonary artery, and this effect was related with PH progression. Conclu‐sion :Plasma MPs level in rats increased gradually with PH disease progression, the increased MPs can impair endo‐thelial function, this finding further perfects the mechanism of PH, and provide a theoretical basis for the future pre‐vention and treatment of PH.%目的：研究循环微粒（MPs）在肺动脉高压（PH）大鼠体内含量的改变及其与肺动脉高压的关系。方法：将48只雄性 Wistar大鼠中30只随机分为对照组、MCT3组（M3组）、MCT5组（M5组）3组，每组10只。M3组、M5组用野百合碱一次性腹腔注射诱导PH模型，剩余的18只用于后续血管环检测。M3组和M5组分别在注射野百合碱后3周（中期）和5周（晚期）时右心导管法测定右心室收缩压（RVSP）来间接反映肺动脉压力，然后用腹主动脉采血法抽取大鼠血液并提取和检测MPs。用提取的三组MPs刺激大鼠肺动脉（每组6只）后检测内皮型一氧化氮合酶（eNOS）、弹性蛋白（elastin）蛋白的变化。结果：大鼠血浆MPs 含量随PH病情进展而逐渐升高（M5＞ M3＞C）；MPs处理肺动脉后可下调eNOS表达，但是上调elastin表达，而且这一作用与PH病情相关，即M5作用强于M3（P＜0．05）。结论：MPs随PH进展逐渐升高，升高的MPs可以损害内皮功能，这一发现进一步完善了PH的发病机制，为将来防治PH提供理论依据。
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