酸枣仁汤对睡眠剥夺大鼠认知功能及海马NA，GABA，5-HT的影响

摘要

目的：观察复方酸枣仁汤（SZRT）对睡眠剥夺（SD）大鼠认知功能及海马NA ，GABA ，5‐HT含量的影响。方法：行为学筛选合格的雄性大鼠50只分为对照组、睡眠剥夺组、酸枣仁汤（SZRT ）21．0g／kg ，10．50g／kg ，5．25 g／kg剂量组，建立睡眠剥夺大鼠动物模型，进行7d连续睡眠剥夺，1d1次，Y‐型电迷宫测定学习记忆，Elisa法检测海马中去甲肾上腺素（N A ），γ‐氨基丁酸（GABA），5‐羟色胺（5‐HT）的含量。结果：与睡眠剥夺组相比，SZRT 21．0g／kg组大鼠的学习记忆的能力改善，NA的含量降低，GABA和5‐HT 含量升高。结论：酸枣仁汤（SZRT ）可改善睡眠剥夺大鼠认知功能，与改变单胺类递质作用有关。%Objective:To investigate the effects of Suanzaoren Decoction on the cognitive function in sleep de‐prived rats and the content of NA ,GABA ,5‐HT in hippocampus .Methods:After screening ,selection behavior qualified in rats ,50 male rats were randomly divided into control group ,sleep deprivation group ,SZRT 21 .0g/kg ,10 .50g/kg ,5 .25 g/kg dose group ,The models of sleep deprived were established with modified multiple platform method for seven days , drugs were given once daily .Determine the learning and memory of rats by Y‐type maze ,determine the the content of NA , GABA ,5‐HT in hippocampus by Elisa .Results:Compared with sleep deprivation group ,SZRT 21 .0g/kg dose group could improve learning and memory ,decrease the content of NA ,increase the content GABA ,5‐HT in hippocampal .Conclusion :Suanzaoren Decoction can improve the learning and memory in rats after sleep deprivation ,may be related to change mono‐amine transmitters .