HPLC法同时测定秦皮中四种成分含量的研究

摘要

目的：建立同时测定秦皮中秦皮甲素、秦皮乙素、秦皮素、秦皮苷的 HPLC法，为秦皮的质量评价提供定量方法基础。方法：色谱柱：Betasil‐C18（250mm ×4．6mm ，5μm ，LOT ：13697），流动相：乙腈‐0．1％磷酸水溶液（13∶87），检测波长：334nm ，柱温：30℃，流速：1ml／min。结果：秦皮甲素、秦皮乙素、秦皮素、秦皮苷分别在0．3090～3．0900μg、0．1275～1．2750μg、0．0276～0．2760μg、0．0540～0．5400μg （ r＝0．999）呈线性关系。结论：本法简单、快速，重现性好，准确度高，色谱峰分离度好。%Objective:To establish a simultaneous determination aesculin ,aesculetin ,fraxin ,fraxetin of HPLC method ,provide a quantitative method for quality evaluation of fraxini cortex .Methods:Chromatographic column :Beta‐sil‐C18 (250 mm × 4 .6 mm ,5μm ,the LOT :13697) ,mobile phase:acetonitrile‐0 .1% phosphoric acid aqueous solu‐tion (13∶87) ,detection wavelength:334 nm ,column temperature:30℃ ,the flow rate:1ml/min .Results:Aesculin , aesculetin ,fraxin ,fraxetin in 0 .3090~3 .0900μg ,0 .1275~1 .2750μg ,0 .0276~0 .2760μg ,0 .0540~0 .5400μg (r=0 . 999) have a good linear relationship .Conclusion:This method is simple ,rapid ,good reproducibility ,high accuracy , good chromatographic peak separation .